Sugar is produced in many countries all over the world. The term “sugar production” relates to various products, primarily mono- and disaccharides.
There are two widely applied large-scale chromatographic carbohydrate (sugar) separations: the isolation of the disaccharide sucrose from molasses and the separation of the two monosaccharides fructose and glucose (figure 1).
Figure 1: The structure of fructose (left) and of glucose (right)
Monosaccharides like fructose and glucose are produced mainly from beet, cane or corn. After several refinery steps including milling, filtration
This mixture is separated by chromatographic separation into a fructose-rich and a glucose-rich component. The dry matter content of the feed flow varies up to 60%w/w.
The resin material used in fructose/glucose fractionation is a gel-type sulfonated polystyrene-DVB strong acid exchange resin in the calcium form. The separation is based on the preferential adsorption of fructose, which forms a complex with the calcium ions. This chromatographic method is called Ligand Exchange Chromatography (LEC). There are several commercially available resins for this application.
A secondary phenomenon is that larger molecules, i.e. higher oligosaccharides are not able to physically fit into the resin pores. The mechanism of separating large molecules from small molecules by preventing some of the large molecules from getting inside the stationary packing is called size exclusion chromatography. Size exclusion chromatography takes place simultaneously with ligand exchange chromatography in the purification of fructose. This results
Both components are recovered for over 90% and the purity of the product flows is above 90% as well. Glucose is recycled and re-isomerized; fructose is sold as a pure product or mixed with the equilibrium mixture mentioned above to yield
The chromatographic separation of fructose and glucose can be efficiently done in a simulated moving bed (SMB). Figure 2 shows experimental data from a glucose-fructose fractionation in
Figure 2.Comparison of production data of fructose/glucose fractionation on a 12-column system with calculations according to our process design model
The same fractionation mechanism applies for lots of other applications,
Figure 3. Representation of a 4-zone SMB system to separate Fructose (strongest chelating) in Extract Phase (yellow exit) from Glucose (weakest chelating) in Raffinate Phase (blue exit). Green input flow represents the feed mixture. The white influent represents the
Figure 3 conveys a generic projection of a 4 zone / 8 column fractionating SMB system., it is
The fractionating principle evidently can be applied in a large field of applications.
In conclusion, Fractionating Chromatography is a strong separation tool that perfectly suits continuous SMB operation.